Transformational Antibacterial Target Validation via Tunable Degradation:
Tunable Target Degradation Platform
- Unprecedented validation of essential & non-essential targets
- Significant advance over genetic-knockouts or anti-sense silencing
- Functional assessment of targets and pathways in vitro and in vivo
- Determines inhibition level required to achieve desired phenotype
- Assesses target vulnerability without need for chemical inhibitor
- Enables “target-based” whole cell screening with TTD-modified strains
Proof-of-Concept Data for Precise Control of Target Steady-State Levels:
- A TTD-modified E. coli Essential Gene Library
- Used to identify highest value biological targets for novel anti-infectives
- Broadly applicable to other bacterial species and strains
Integration with MINE-AI Systems Biology platform has potential to redefine antibacterial drug discovery

