Transformational Antibacterial Target Validation via Tunable Degradation:

• Unprecedented validation of essential & non-essential targets
  
• Significant advance over genetic-knockouts or anti-sense silencing
  
• Functional assessment of targets and pathways in vitro and in vivo
• Determines inhibition level required to achieve desired phenotype
• Assesses target vulnerability without need for chemical inhibitor
• Enables “target-based” whole cell screening with TTD-modified strains
  

Proof-of-Concept Data for Precise Control of Target Steady-State Levels:

• A TTD-modified E. coli Essential Gene Library
• Used to identify highest value biological targets for novel anti-infectives
• Broadly applicable to other bacterial species and strains
  

Integration with MINE-AI Systems Biology platform has potential to redefine antibacterial drug discovery